BL4820 Biochemical Techniques

 

 

 

 

 

 

 

 

 

 

 

 

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Reports

Course Philosophy and Expectations

Students will be responsible for their own laboratory reports. Collaboration with your lab partner is expected; however, you should do individual work on your reports. Professional behavior is expected in the laboratory at all times. Grades will be based on laboratory reports and participation.

Syllabus

 

 

 

Week

Dates

Recitation Subject

Laboratory Experiment

1

01.17.08

Introduction

Safety and Orientation

2

01.24.08

Where to find techniques - Spectrophotometry review

L1 Extinction coefficients/Protein assays

 

 

3

01.29.08

01.31.08

Decoding materials and methods

L2 Isolation of salivary amylase

4

02.05.08

02.07.08

WINTER CARNIVAL - NO LAB

5

02.12.08

02.14.08

Purification tables

L3 Electrophoresis/Immunotechniques

6

02.19.08

02.21.08

Writing a research paper

L4 Isolation of avidin from egg white

7

02.26.08

02.28.08

Binding interactions

L4 Binding of biotin to avidin

8

03.04.08

03.06.08

Purification protocols

L5 Computer modeling of HRP

9

03.11.08

03.13.08

SPRING BREAK - NO LAB

10

03.18.08

03.20.08

Kinetics and Inhibition

L5 Isolation of HRP

11

03.25.08

03.27.08

Analysis of draft introductions

L5 Isolation of HRP - chromatography

 

12

04.01.08

04.03.08

Presenting Data

 

L5 Kinetics of HRP

13

04.08.08

04.10.08

Inhibition and stability

L5 Inhibition of HRP

14

04.15.08

04.17.08

Work on Final Report

L5 Enzyme stability

15

04.22.08

Biochemistry Jobs

Make up lab, Drafts Due

 

16

05.01.08

FINALS WEEK - NO CLASS - FINAL REPORTS DUE