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The Wall - Extracellular Proteoglycans

Extracellular Proteoglycans of the Diatom Achnanthes longipes are Organized into Biocomposite Adhesive Structures by a Cell-Directed Self-Assembly Process

Introduction

Significance

Adhesion Sequence of A. longipes

Colloidal gold conjugated monoclonal antibody labelling of intracellular vesicles and Golgi

Anion exchange chromatography of A. longipes WIBS extracellular adhesives

Autofluorescence of Achnanthes longipes extracellular matrix

Cryo-FESEM together with serial section data, indicated extrusion of polymer through frustule poroids

Cryo-FESEM of extracellular adhesive stalks

Cryo-SEM techniques

Proposed structure for the primary A. longipes fucoglucuronogalactan

Summary of antibody localization patterns of A. longipes

Monoclonal Antibody Cell Staining

Reversible inhibition of stalk production in A. longipes by DCB and CBF

SDS-PAGE of cellular proteins from Achnanthes

Size Exclusion Column Chromatography

Model of the stalk of A. longipes detailing the location of components characterized here and putative sites of the origin from the frustule

Interaction of antibodies with water insoluble/bicarbonate soluble (WIBS) portion of adhesives and fractions or modifications thereof

Summary and Conclusions

Publications

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This page Copyright © 2001 Michael Gretz
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