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The Wall - Diatom Synthesis

Synthesis and Assembly of Extra Cellular Adhesives of the Marine Fouling Diatom Achnanthes Longipes

Abstract

Introduction

Results

Results and Discussion

Materials and Methods

Figure 1 - Protocol for chemical isolation and fractionation of extracellular polymers

Figures 1-34

Figures 1-34 (description)

Figure 2 - Adhesion sequence of A. longipes

Figure 35 - Monosaccharide and linkage/substitution site profiles of Achnanthes mechanically isolated extracellular adhesives and lectin-FITC staining results.

Figure 36 - Antibody localization and corresponding light microscope images of Achnanthes extracellular adhesive.

Figure 37 - Reversible inhibition of Achnanthes stalk production by DCB and CBF.

Figure 38 - SDS-PAGE of cellular proteins from Achnanthes labelled with fluorescent DCB analog (DCBF, lane 2) and Coomasie blue (lane 3), 8µg/lane.

Table 1 - Localization of Achnanthes EPS using (1a) FITCconjugated lectins and (1b) antibodies derived from cloned and uncloned mouse hybridoma cell lines.

Diagram of Table 1

Assorted figures (Photo)

Acknowledgements

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