BL/CH 401 Lecture #5 - Protein Primary Structure

Edman Method for Peptide Sequencing

Finally, after each peptide is separated into a pure form...

from the mixture of peptides generated by the cleavage method,

the amino acid sequence of each peptide is determined using the Edman Reagent:

Phenylisothiocyanate

It works by cleaving the N-terminal AA off and leaving the rest of the peptide intact.

{*Figure 16*}

The Edman method can be repeated on the peptide remaining after the reaction,

so this is a very effective method for obtaining AA sequence information.

In fact the whole sequence of a peptide containing many AAs can be determined by Edman method.

Go To: AA Sequence Help for more info on Edman degration

©Wilbur H. Campbell, 1995, 1996; wcampbel@mtu.edu

Back To: Lecture 5 - Protein Primary Structure