|
BL4820 GOT Purification Summary
PDF File for Printing: Examples of GOT Summary
Tables
A. Purification Steps
- I. Crude Extract of Heart (FI)
- II. Heated Supernatant (FII)
- III. Ammonium Sulfate Concentrate/Dialyzed (ASII)
- IV. Carboxy-Methyl Cellulose Ion-Exchange Salt Elution
(CMC-14)
- V. "Summed" CMC Fractions
B. Results from Fall 1993
Group A
| Purification Step |
Protein |
GOT |
Total GOT |
Specific Activity |
Yield |
Fold-Increase |
| |
mg/ml |
Units/ml |
Units |
Units/mg |
% |
|
| |
|
|
|
|
|
|
| I. (Vol = 45 ml) |
18 |
30 |
1350 |
1.6 |
100 |
1 |
| II. (Vol = 44 ml) |
9.6 |
19 |
840 |
2.0 |
62 |
1.2 |
| III. (Vol =2.8 ml) |
10 |
283 |
790 |
28.3 |
59 |
18 |
| IV. CMC 14/5ml |
0.22 |
13 |
65 |
60 |
5 |
38 |
Group B
| Purification Step |
Protein |
GOT |
Total GOT |
Specific Activity |
Yield |
Fold-Increase |
| |
mg/ml |
Units/ml |
Units |
Units/mg |
% |
|
| |
|
|
|
|
|
|
| I. (Vol = 45 ml) |
24 |
31 |
1400 |
1.3 |
100 |
1 |
| II. (Vol = 48 ml) |
2.8 |
30 |
1440 |
11 |
103 |
8.5 |
| III. (Vol =2.5 ml) |
14 |
330 |
825 |
24 |
59 |
18 |
| IV. CMC 14/5ml |
0.15 |
13 |
65 |
87 |
5 |
67 |
| V. Sum CMC-13 to CMC-19 |
------- |
------- |
225 |
------ |
16 |
------- |
Group C
| Purification Step |
Protein |
GOT |
Total GOT |
Specific Activity |
Yield |
Fold-Increase |
| |
mg/ml |
Units/ml |
Units |
Units/mg |
% |
|
| |
|
|
|
|
|
|
| I. (Vol = 57 ml) |
16 |
35 |
1995 |
2.1 |
100 |
1 |
| II. (Vol = 44 ml) |
1 |
28 |
1230 |
28 |
62 |
13 |
| III. (Vol =2.5 ml) |
2.8 |
63 |
160 |
23 |
8 |
11 |
| IV. CMC 14/5ml |
0.031 |
2.65 |
13 |
85 |
0.7 |
40 |
| IV. CMC-15/5ml |
0.036 |
2.56 |
13 |
71 |
0.7 |
34 |
Back To:
BL4820 Lab Schedule
©Wilbur H. Campbell, 1996; wcampbel@mtu.edu
|