BL4820 Biochemistry Techniques

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Lab Schedule for SPRING 2003

Lecture: Tues 12-1 PM in EERC Rm 315

Lab: Wed 2-5 PM and Thurs 9-Noon in "Biochem Lab" (ESE 711)

Week in Term and Lecture Date

Text Expt

Topic

1. Jan 14 Lecture 1 1 Protein Assay and Standard Curve (View Lab Safety Video)
2. Jan 21 Lecture 2 9 Enzyme Assay & pH Optimum for Acid Phosphatase
3. Jan 28 Lecture 3 9 Enzyme Kinetics for Acid Phosphatase
4. Feb 4 Lecture 4 10 Glutamate Oxaloacetate Transaminase (GOT) Purification

A. Initial Purification and Assays

5.  Feb 11 No lecture Winter Carnival    
6. Feb 18 Lecture 5 10 B. GOT - Ammonium Sulfate Fractionation & Dialysis
7. Feb 25 Lecture 6 10 C. GOT - Ion Exchange Chromatography

Examples of Summary Table for GOT Purification

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Copyright ©1996-2003 Wilbur H. Campbell, All Rights Reserved; wcampbel@mtu.edu

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