BL4820 Biochemistry Techniques

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Lab Schedule for SPRING 2002

Lab: Wed 2-5 PM and Thurs 9-Noon in "Biochem Lab" (ESE 711)

Week in Term and Date

Text Expt

Topic

1. Jan 14 1 Protein Assay and Standard Curve (View Lab Safety Video)
2. Jan 22 9 Enzyme Assay & pH Optimum for Acid Phosphatase
3. Jan 28 9 Enzyme Kinetics for Acid Phosphatase
5. Feb 11 10 Glutamate Oxaloacetate Transaminase (GOT)

A. Initial Purification and Assays

6. Feb 18 10 B. GOT - Ammonium Sulfate Fractionation & Dialysis
7. Feb 25 10 C. GOT - Ion Exchange Chromatography

Examples of Summary Table for GOT Purification
8. Mar 11 4 GOT Native Gel Electrophoresis for Enzyme Purity and GOT Denaturing (SDS-PAGE) Gels for Subunit Molecular Weight and Gel Filtration Chromatography for Native MW of GOT

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