483 Lecture 6 - Glutamate Oxaloacetic Acid Transaminase (GOT) Purification -- Expt 3
Part C
Lab Report for Expt #3 - What to turn in for this Lab Report
Here is a list of what you need to turn in for your Lab Report - use the usual 4 part
style: Introduction, Methods, Results, and Discussion.
In the Results be sure to include the following:
- Flow Chart showing how the Purification was done and here define the
Abbreviations to be used for various steps - for example, define F-I as Fraction I - Pig
Heart Centrifuged Crude Extract. If you do not define the various abbreviations like F-I,
F-II, AS-I, AS-II, AS-III, CMC etc., then the report is confusing and incomplete. Think
about your Lab Report as being read by someone who is not as familiar with the procedure as
you expect me to be.
- GOT rates for NADH based activity assays for all fractions analyzed and calculations
illustrating how these were converted to GOT Activities as described in Part 3 of these lecture notes.
- GDH rates for assays to detect contamination by this enzyme
- Protein Assay results, including standard curve for Folin assay (identify standard
protein used), volumes of fractions, dilution of these fractions, absorbances for the
assay results and calculations of concentration of protein in the fraction in mg/ml.
- Graphic for CMC column elution as illustrated in Part 5 of
these lecture notes.
- Purification Summary Table, as illustrated in Part 4 of these
lecture notes.
Back To: Lecture 6 - Week #6
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