483 Lecture 6 - Glutamate Oxaloacetic Acid Transaminase (GOT) Purification -- Expt 3 Part C
5. Graphic Illustrating the CMC Purification Step
You must make a plot of the elution of the CMC column where you plot two parameters vs. fraction number:
- Absorbance at 280 nm for each fraction
- GOT Activity in Units/ml (ie. µmol OAA formed/min/ml)
This is a bit of a complicated graphic and so you should make it by hand using at least 1/2 a sheet of graph paper to make it sufficiently large to easy read. Be sure to use different Y-axis for each of the parameters as well as different symbols to plot them.
Here is an example graphic like the one you should put in your Lab Report:
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