BL4820 Biochemistry Techniques

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Safety Rules

The following information on laboratory safety are "THE RULES" for this lab and do not apply to other courses in the Dept. of Biol. Sciences. However, developing and maintaining safe lab practice is essential for your future and these guidelines may be useful to you in the future.

1. LAB SAFETY VIDEO: A short video will be shown at the beginning of your first lab.

ALL STUDENTS MUST SEE THIS VIDEO PRIOR TO STARTING ANY EXPERIMENTS

2. Eating or drinking or smoking are not allowed in the lab.

3. Mouth pipetting is not allowed! USE pipetters or pro-pipets with glass pipets at all times.

4. HAZARDOUS CHEMICALS:

Many of the chemicals used in this course constitute a hazard to you; therefore, you must be aware of these hazards before you begin to handle chemicals. The lab text identifies those chemicals with the greatest hazard in an experiment. In my lectures I will also call your attention to hazardous chemicals being used in that week's experiment. In general, you will be using solutions of acids and bases and you should treat them with appropriate caution.

5. EYE PROTECTION: The use of safety glasses is required in this course and you must provide them.

6. SAFETY DEVICES: A fire extinguisher is located near the door in the lab and you should be sure you know where it is and how to use it. A safety shower is located in the lab and you will be shown the location at the beginning of the first lab. The fire alarm and emergency exits are in the hall and these will be identified for you in the first lab session.

7. LAB CLOTHING: Lab coats are not required for this course, but are recommended. Long hair must be constrained and loose clothing is not allowed. A near-boiling water bath is used in Expt 3 in the labs during Week 4; special care should be used to avoid buring yourself with the hot water.

8. BROKEN GLASS: IF you break glassware or pipets, do not use them further. Place broken glass in the container maker "GLASS WASTE". Do not put broken glass in the normal waste container.

9. WASTE DISPOSAL: All liquid wastes are to be poured down the drain in the main lab sink while running the water.

10. NO ONE IS ALLOWED TO WORK ALONE IN THE LAB!!! All experiments are to be conducted in the lab and in the alloted lab periods. The TA or I will be present at all times. You can not work in this lab at any other time. If a lab needs to be made up due to an excused absence, I will arrange for you to meet with a different lab section.

IN AN EMERGENCY CALL: MTU PUBLIC SAFETY -- 2216 -- USE PHONE IN HALL

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