EXAM II - Answers
1. Kinetics Problem - 50 Points.
Km = 0.2 mM
Vmax = 75 micromol/min
Inhibitor A = Non-Competitive
Km' = 0.2 mM
Vmax' = 25 micromol/min
At [I] = [A] = 10 microM, Ki = 5 microM.
Inhibitor B = Competitive
Km' = 0.4 mM
Vmax' = 75 micromol/min
At [I] = [B] = 300 microM, Ki = 300 microM.
2. Protein Structure - 40 Points.
See lecture notes for Lectures 8 and 9.
3. Thought Problem - 10 Points.
Methanol and ethanol are both substrates for alcohol dehydrogenase. So, when ethanol is given to a person with methanol poisoning, ethanol will act as a competitive inhibitor and thereby prevent (at least dramatically slow) the catalytic conversion of methanol to formaldehyde, which presumably causes the damage in methanol poisoning.
©Wilbur H. Campbell, 1995; wcampbel@mtu.edu