EXAM II
1. Kinetics Problem - 50 Points.
A. Make Plots of i) Vo vs. [S] and ii) 1/Vo vs. 1/[S] -- Using graph paper provided.
B. Determine the Km, Vmax, type of inhibitors for A and B, and Ki values for inhibitors A and B (give proper Units).
Km' = Km (1 + [I]/Ki)
Vmax' = Vmax/(1 + [I]/Ki)
[S] Vo (µmol/min)mM No Inhibitor I = A = 10 µM I = B = 300 µM
0.00 0.0 0.0 0.0
0.05 15.0 5.0 8.3
0.10 25.0 8.3 15.0
0.20 37.5 12.5 25.0
1.00 62.5 20.8 53.6
5.00 72.1 24.0 69.4
2. Protein Structure - 40 Points.
Fully describe with words and illustrate with a small graphic:
A. The four levels of Protein Structure
B. The types of bonding that stabilize these parts of a protein
3. Thought Problem - 10 Points.
Explain briefly why giving ethanol to a person who has been poisoned with methanol will be beneficial and perhaps prevent the damage normally caused by methanol (ie. blindness). Your explanation/answer must be in biochemical terms to get the credit.
(Hint: think about the chemical structures of the two alcohols).
©Wilbur H. Campbell, 1995; wcampbel@mtu.edu