{*Figure 18*}Protein/Enzyme Purification -- Part II
Section C
Native MW is estimated by gel filtration and...
used together with subunit MW to figure out the subunit composition of the enzyme/protein.
Estimating Native MW:
{*Figure 18*}
Plot of the calibration for a Gel Filtration Column.
The MW of an unknown protein can be estimated using this plot.
Since proteins/enzymes are usually not symmetric,
Gel Filtration provides only an approximate MW.
For more accurate MW determinations, more sophisticated methods must be used.
But these other methods will not be discussed in this course.
(Figure 18 is from Lenhigher Biochemistry ©19?? Worth)
©Wilbur H. Campbell, 1995; wcampbel@mtu.edu