Protein/Enzyme Purification -- Part II
Section A
NATIVE PAGE
The proteins are maintained in their Native, Active form during Native PAGE
Then after electrophoresis the gel can be stained for enzyme activity and for protein content.
Native Gels to test Purity:
After ion-exchange column chromatography (DEAE cellulose, for example), the enzyme/protein is usually not completely pure:
{*Figure 14*}
The enzyme activity stain reveals the position of the enzyme after PAGE, while the total protein stain reveals the position of all the proteins on the gel after PAGE
{*Figure 15*}
If you find only one band of protein, it is pure and...
called a homogeneous protein or enzyme.
©Wilbur H. Campbell, 1995; wcampbel@mtu.edu