{*Figure 5*}
Protein/Enzyme Purification -- PART I
Section C
For protein binding, the pH is fixed near neutral at a very low salt concentration:
{*Figure 5*}
Then add increasing amounts of salt to release protein:
{*Figure 6*}
Illustration of how column chromatography is done with a mixture of proteins (colored zones):
{*Figure 7*}
As the high salt solution passes over the bed of cellulose particles with the protein mixture bound to it, individual proteins (represented by colors) are separated and collected in test tubes in sequence.
Protein concentration in the tubes is determined (by reading absorbance in UV with spectrophotometer) and the protein concentration is plotted against fraction number or volume of elution from column.
[Figure 7 from Voet & Voet Biochemistry Text © 1990 John Wiley & Sons]
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©Wilbur H. Campbell, 1995; wcampbel@mtu.edu