Enzyme Kinetics -- Part I
Part II. A Simple Mechanism for the Enzyme Catalyzed Reaction.
For catalysis to begin, the substrate must bind to the enzyme, which results in the formation of the enzyme-substrate complex (ie E-S complex). The E-S complex forms rapidly in the first part of the enzyme catalysis process and the concentration of the E-S stays constant at a steady-state level. For this reason, this type of kinetics is called steady-state kinetics.
A simple mechanism for the enzyme catalyzed reaction helps us to understand and model this process.
Figure 2. A simple enzyme mechanism for a single substrate and product.
This simple mechanism can also be expressed with rates shown for each process in the mechanism:
Figure 2a. Simple enzyme mechanism with rate constants shown for each step.
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©Wilbur H. Campbell, 1995; wcampbel@mtu.edu