BL/CH401
EXAM 2 Scoop

BL401/CH401

Exam II

October 22, 1993

1. Kinetics Problem --- 50 Pts

A. Make Plots of i) vo vs. [S] and ii) 1/vo vs. 1/[S]

B. Determine the Km, Vmax and Ki values for inhibitors A and B (give proper Units)

Km' = Km (1 + [I]/Ki)

Vmax' = Vmax/(1 + [I]/Ki)

Figure 1. Data for Kinetics Problem.

2. Protein Structure ---- 40 Pts.

Briefly describe and illustrate with a small graphic:

A. The four levels of Protein Structure

B. The types of bonding that stabilize these parts of a protein

3. Thought Question ---- 10 Pts.

Explain briefly how a single amino acid change in the sequence of a protein can cause a disease. For example, sickle cell anemia.