EXAM 2 Scoop
Biochemistry I -- EXAM II
Oct. 21, 1992
1. 50 Pts. Using the data shown below --
1) Draw "vo vs. [S]" and "1/vo vs 1/[S]" plots
2) Determine the Km and Vmax from the double reciprocal plot
3) Decide what type of inhibitors "x" and "z" are
4) Calculate the Ki for "x" and "z"
Competitive Inhibitor : Km' = Km (1 + [I]/Ki)
Noncompetitive Inhibitor: Vmax' = Vmax / (1 + [I]/Ki)
Figure 1. Data for kinetics problem
2. 40 Pts. Briefly describe each of the four levels of protein structure and illustrate the type of bonding or other stabilizing force involved in each type.
For your illustrations provide a drawing representing the chemical bonding involved with enough detail to show me that you know what you are describing.
BE SURE TO PUT DOWN ENOUGH INFORMATION TO GET FULL CREDIT!!!
3. 10 Pts. In discussing the relationship between a protein's structure and its function, I identified 3 types of situations that we now know. BASED ON THE INFORMATION I PROVIDED IN CLASS VERY BRIEFLY ANSWER THE FOLLOWING:
1) List these three types of proteins
2) Provide the features of each type
3) What were the 3 example proteins or types of 3-D shape
©Wilbur H. Campbell, 1995; wcampbel@mtu.edu